Victor M. Lasehinde

Optimized the laboratory's chromatin immunoprecipitation (ChIP) assay protocols, establishing robust methods for crosslinking, sonication, antibody selection, and qPCR validation that remain in active use.

Provide ongoing technical guidance to a Staff Scientist on ChIP experimental troubleshooting, reagent validation, and protocol refinements, significantly improving data quality and reproducibility.

Developed and shared updated procedural documentation and virtual demonstrations, standardizing ChIP workflows and facilitating consistent execution and training for new personnel.

Collaborate on interpreting complex ChIP-qPCR and sequencing results, advising on normalization strategies, control validation, and statistical analysis to ensure rigorous scientific interpretation.

Optimized scalable nucleic acid extraction and purification workflows, improving throughput and reliability for administrative processes, workflow expediting, and operational efficiency.

Executed epigenetic analyses and developed molecular diagnostic assays using ChIP, qRT-PCR, and dual luciferase, directly contributing to 4 co-authored publications in high-impact journals (Mol Cell, JBC).

Characterized stress-induced protein and RNA expression changes using Western blotting, Northern blotting, and SDS-PAGE, enabling quantitative pathway activation analysis and sample quality assurance.

Engineered custom plasmids for gene expression studies via molecular cloning and validated subcellular localization and protein interactions using immunofluorescence microscopy and cell sorting.

Processed and analyzed experimental data, quantifying gel images with Image Quant, performing statistical analysis in GraphPad Prism, and creating publication-ready figures using Adobe Photoshop and Illustrator.

Managed end-to-end laboratory operations for NIH-funded projects, reducing annual operational costs by $65K (20%) through strategic vendor management and supply chain optimization.

Maintained audit-ready, GLP-compliant documentation, managed equipment and supply inventories, and oversaw sample logistics, ensuring compliance and operational readiness.

Conducted patient safety assessments and managed the collection, processing, and chain-of-custody for 100+ clinical samples under ICH-GCP guidelines.

Applied knowledge of GCP in writing a co-authored publication (Cureus) related to studies in hidradenitis suppurativa and atopic dermatitis.

Developed cell-based assays using high-content imaging and quantitative image analysis to assess compound efficacy and cytotoxicity for antidiabetic drug candidates, contributing to a co-authored publication in Frontiers in Microbiology.

Pioneered robust sample preparation protocols for novel renal injury biomarkers (KIM-1), creating workflows for downstream analytical characterization via Western blotting, ELISA, and SDS-PAGE.

Synthesized complex datasets using GraphPad Prism, Minitab, and JMP for statistical analysis, and leveraged Adobe Photoshop & Illustrator for professional figure creation.

Authored and contributed to research grant applications, SOPs, and technical reports, ensuring comprehensive documentation of methodologies and findings.

Spearheaded operational execution of a multi-year clinical trial for diabetes management, orchestrating all phases from protocol implementation to data lock, involving 300+ patient volunteers and 1,000+ clinical visits.

Bridged clinical data with molecular insights by processing and analyzing 200+ patient serum and tissue samples, quantifying pharmacodynamic biomarkers, and linking patient responses to mechanistic pathways.

Served as primary custodian of study quality and ethics, ensuring strict adherence to ICH-GCP and HIPAA-equivalent regulations, developing and maintaining an audit-ready master file of essential documents.

Analyzed integrated clinical endpoint and experimental biomarker data using Microsoft Excel to evaluate therapeutic efficacy, generate hypotheses, and author interim reports translating patient outcomes into actionable insights.

Conducted direct patient assessments to identify and triage adverse events, mentoring colleagues on standardized classification, and ensuring timely, accurate reporting to the institutional review board.